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Journal : Jurnal Veteriner

Sebaran Karbohidrat pada Kelenjar Ludah Biawak Air (Varanus salvator) (DISTRIBUTION OF CARBOHYDRATES IN THE SALIVARY GLANDS OF WATER MONITOR (VARANUS SALVATOR)) Idawati Nasution; Alfajri Saputra; Hamny .; Muhammad Jalaluddin; Sri Wahyuni
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to determine the carbohydrate content of salivary glands lingual,sublingual, and mandibular glands of water monitor (Varanus salvator). This studywas used the lingualglands, sublingual, and mandibularof an adult male water monitor. Salivary glands were fixed by soakingin a solution of 4% paraformaldehyde for four days. Then, the tissuewas processed into routine histologicalpreparations. Salivary gland tissue was stained with alcianblue staining (AB) pH 2.5 for detecting acidiccarbohydrates and was stained with periodic acid Schiff (PAS) for detecting neutral carbohydrates. Theresults showed that the lingual and sublingual glands showed a positive reaction to AB pH 2.5 stainingand PAS with the intensity varied from weak (+) to strong intensity (+++) scattered in the cytoplasm of thecell. In the cytoplasm of cells of the mandibular glands showed negative reaction (-) to the AB pH 2.5staining but reacted positively to PAS with the intensity varied from weak (+) to strong (+++). Based onthe result of the study it can be concluded that lingual and sublingual glands of Water monitor containacidic and neutral carbohydrates while mandibular glands only contain neutral carbohydrates.
CARBOHYDRATES OF CHANGES DURING THE FOLLICULAR DEVELOPMENT IN THE OVARY OF THE MOUSE DEER, TRAGULUS JAVANICUS Hamny -; Srihadi Agungpriyono; Ita Djuwita; Chairun Nisa; Wahono Esthi Prasetyaningtyas; Erdiansyah Rahmi
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The data available on the female reproductive organ of mouse deer (Tragulus javanicus) is still very limited. A study was therefore conducted to investigate the distribution and the concentration of carbohydrate residues during the development of ovary follicles. An ovary at luteal phase was used in this study. Thin sections of the ovary were prepared occording to the standard methods and they were then histochemically stained with flourecnece-labelled lectins such as peanut agglutinin (PNA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), Winged bean agglutinin (WGA) and Ulex europaeus agglutinin (UEA). The result showed that changes in the distribution and the concentration of carbohydrate occured during the development of the follicle. During the preantral stage, the cytoplasm of oosit contained carbohydrate with the residues of glucosa dan mannosa. Zona pelusida contained carbohydrates with residues of glucosa, mannosa, galactosa dan N-asetylgalactosamine, whereas extracellular matrix contained carbohydrate with the residues of glucosa dan mannosa. In the antral follicle, the cyitoplasm of oocytes contained carbohydarte with the residues of galactosa dan N-asetylgalactosamine, whereas its zona pelusida, extracellular matrix and follicular fluid contained carbohydarte with the residues of fucosa, N-asetylglucosamin and cyalic acid. Diffrences in the types and the distribution pattern of carbohydrates were observed in this study, both in preantral and antral follicles.
Morfologi Anatomi dan Histologi Usus Biawak Air (Varanus salvator) THE ANATOMICAL AND HISTOLOGICAL MORPHOLOGY OF INTESTINAL WATER MONITOR (VARANUS SALVATOR) Hamny .; Sri Mulyani; Dian Masyitha; Sri Wahyuni; Muhammad Jalaluddin
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to determine the anatomical morphology and histology of the intestineof water monitor (Varanussalvator). Intestine organ from an adult male water monito was used in thisstudy. Perfusion was performed to flow the 4% paraformaldehyde fixative solution through the heart.Intestine was collected, then divided into six sections, followed with dipping them into 4% paraformaldehydesolution for four days, continued histological preparations and stained with haematoxylin eosin (HE). Theanatomical morphology observation was performed before the intestine was processed into histologicalpreparations by observing the outer shape of the intestine and measuring it’slength. Morphologicalobservation was made †after processing theintestinal tissue by observing each portion of the intestinalwall structure. The results showed that transverse fold were found on the outer and inner surfaces of theintestine section I-IV, while in the V-VI part of the intestine, the folds were not observed anymore. Theintestine wall consisted of four layers, such as mucosa, submucosa, tunica muscularis, and serosa. Thevilli on intestine section I-IV were relatively longer and higher, while the intestine vili in section V-VI wererelatively short. The entire mucosa in water monitor was coated with absorptive columnar epitheliumwith goblet cells. Based on the result study, it can be concluded that the water monitor has more efficientdigestive process which can be seen morphologically from the relatively short intestinal, has transversefolds on the surface of the small intestine, and does not have cecum. The intestinal wall consists of layersi.e : mucosa, submucosa, tunica muscularis, and serosa.
Polimorfisme Gen Aquaporin-3 dan Pengaruhnya Terhadap Ikatan dengan Ligand Uji Secara In Silico Hamny Sofyan; Noni Zakiah; Hasriati Hasriati; Frengki Frengki; Taufan Hidayat; Siti Aisyah
Jurnal Veteriner Vol 23 No 2 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.8 KB) | DOI: 10.19087/jveteriner.2022.23.2.157

Abstract

Changes in the genetic code can trigger changes in the function of the proteins it produces. Genetic changes that commonly occur in the form of single nucleotide polymorphism such as in the aquaporin group gene (AQPs) which play an important role in maintaining moisture and elasticity of the skin epidermis. This study aims to analyze the effect of mutations in one of the aquaporin protein groups (AQPs), namely aquaporin 3 (AQP3) by in silico. Research material in the form of data sequences and 3D structures of AQP3 wild-type dan AQP3 mutant downloaded from the protein databank (pdb id 1FX8 and 1LDF). These two sequences were aligned using the “ClustalW” method to observe the position of the mutation, while the 3D structures were also aligned using the “Alignment” method to observe changes in the native ligand’s “site binding” region. The impact of the conformational changes of mutant AQP3 was also observed on several compounds as test ligands which are known to work to support the function of AQP3 protein such as asiatic acid, madecassic acid, asiaticoside, and alpha-retinoic acid through molecular docking method. Sequence alignment results showed that mutations occurred in the 48th (tryptophan-phenylalanine) and 200th (phenylalanine-threonine) residue sequences. The mutation of the two residues caused a change in the interaction model of all test ligands compared to the normal type of AQP3. The mutation also causes a decrease in the stability of AQP3, but on the contrary does not change the function of AQP3 but only decreases the binding ability of the native ligand molecule which has implications for the decrease in the binding ability of water molecules, glycerol and the test ligand.
Co-Authors . Nurliana . Zuchri Abdul Harris Abdullah Hamzah Ahmad Azhari Nopiosi Alfajri Saputra Alfajri Saputra Aliman Irfandi Amalia Sutriana Amiruddin A Anugrah Septian Aris Junaidi Ayu Agita Ginting Bambang Pontjo Priosoeryanto Boni Anggara Budianto Panjaitan Budianto Panjaitan Chairun Nisa Chika Nahara Ramadhanty Chintya Desfariza Cut Dahlia Iskandar Cut Dahlia Iskandar Darmawi Darmawi Debby Novita ayumi Dian Masyitha Dian Masyitha Dwinna Aliza Erdiansyah Rahmi Erdiansyah Rahmi Ezy Yulanda Rezki Fadli A Gani Fadli A. Gani Fadli A. Gani Fadli A. Gani Fadli. A. Gani Faisal Jamin Fakhrurrazi Fakhrurrazi Fakhrurrazi Fakhrurrazi Fitriani Fitriani Fitriani Fitriani Frengki Frengki Gholib Gholib Hafizuddin Hafizuddin Hamdani Budiman Hasriati Hasriati Hendri Hermawan Adinugraha Herlina Yuliani Herrialfian Herrialfian I. Djuwita . I.Nasution . Idawati Nasution Idawati Nasution Idawati Nasution Idawati Nasution Ismail Ismail Ismail Ismail Ita Djuwita Ita Djuwita Joharsyah J Juli Melia Ketut Adnyane Mudite Lailia Dwi Kusuma Wardhani Lailia Dwi Kusuma Wardhani Lidya Elizabeth M. Manik M. Hasan M. Isa Mahdi Abrar Mahdi Abrar Manfarizah Manfarizah Maryulia Dewi Mudhita Zikkrullah Ritonga MUHAMMAD AGIL Muhammad Hambal Muhammad Hambal Muhammad Iqbal Muhammad Jalaluddin Muhammad Jalaluddin Muhammad Jalaluddin Muhammad Zulfan Mulyadi Adam Mulyadi Adam Mulyadi Adam Muslim Akmal Muslim Akmal Mustafa Sabri Mustafa Sabri Mustafa Sabri Nabila Latifa Hafizsha Nadia Hanifah Nanda Yulian Syah Nauval Gibran Lubis Noni Zakiah Nurhidayat, Nurhidayat Nuzul Asmilia Patrick Flaggellata Prabowo Purwono Putro Primaadhi Abimanyu Satrio Raharjo Rastina Rastina Razali Daud Rinidar R Rinidar Rinidar Roslizawaty R S. Agungpriyono . Safni Kamal Savitri Novelina Shinta Mutia RM Siti Aisyah Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Sri Wahyuni Srihadi Agungpriyono Sugito Sugito Sulasmi Sulasmi Suriadi S Syafruddin S Syafruddin Syafruddin Syakur Syakur T. Armansyah T. Armansyah T. Armansyah, TR Taufan Hidayat Taufan Hidayat Taufan Hidayat Taufan Hidayat Teti Arabia Teuku Reza Ferasyi Tongku Nizwan Siregar Triva Murtina Lubis Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Ummu Balqis W.E. Prasetyaningtyas . Wahono Esthi Prasetyaningtyas Wahyuni, Sri Wasmen Manalu Yusnizar Yusnizar Zainabun Zainabun Zainabun Zainabun Zainuddin Z Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zainuddin Zuhrawati Zuhrawati Zuraidawati Zuraidawati